Is the activity of the enzyme protein phosphatase 1 decreased in cancer cells?
Here is what I need the paper to have the protein that I have choose is P53 protein . the task is given and try your best with it and please contact me if you need more info
here is what you need to fallow :
RESEARCH PROPOSAL
General tips
A properly prepared research proposal clearly states the objective(s) of your research (poses the
question), provides some background information that explains why your question is important,
clearly describes your experimental design (the steps you will follow and methods you will use),
and indicates expected outcomes of these steps or experiments based upon your background
knowledge and sound biochemical judgment. Background reading can include your textbook, the
internet or other sources you can find. An alternative hypothesis will be included because your
results may not be as predicted. In the end, a brief summary of follow-up experiments is
proposed, should your results not be as expected.
Proposal Outline
1. Question: Clearly state a question to which you do not know the answer. The question must
be answerable scientifically and, for this particular proposal, must be answerable using one or
more biochemical methods. The biochemical methods need not necessarily be the methods we
used in class. Your question might or might not have already been answered in a published
article. This exercise is not designed to test how well you are acquainted with the scientific
literature but how well you can design experiments to clearly answer a question based on your
scientific understanding, logic, and scientific originality. Do not plagiarize! Plagiarism is “to
use, and pass off as one’s own, someone else’s writing or speech”. A plagiarized report will
result in a lower grade for the entire course.
2. Statement of Purpose (Title): The title is put in the form of a statement rather than a
question. Thus, question: Is the activity of the enzyme protein phosphatase 1 decreased in
cancer cells? Statement (title of proposal): Activity of protein phosphatase 1 in cancer cells.
3. Background Information: Imagine what a reader of your proposal needs to know, in order to
understand the context of your question and why it is important. Include all sources you used
for your information in parenthesis in the text either by the number the source has in your
reference list at the end of the proposal or by the name of the author(s) and the year of
publication.
4. Hypothesis: The expected outcome of your proposed experiments. Example: The activity of
the enzyme protein phosphatase 1 is decreased in cancer cells.
5. Alternative Hypothesis: A possible different outcome of your proposed experiments.
Example: The activity of the enzyme protein phosphatase 1 is unaffected in cancer cells.
6. Experimental Design: Indicate step-by-step specifically what methods you are going to use
and what particular results you expect from each step or method based on your hypothesis.
7. Follow-up Experiments: Briefly describe the experiments you think appropriate, if the
outcomes of your research are not as expected, for example if your hypothesis were not true.
8. References: List all sources you used in the Background Information part or the Experimental
Design part, but include here enough information so that the reader can find the source.
for the method you need to use try to choose one of these (Identification of Serum Glycoproteins by SDS-PAGE , Ion Exchange and Size Exclusion Chromatography ,gel filtration, isometric foc )
